HIGH-THROUGHPUT GENE ANALYSIS USING SUSPENDING DNA FIBERS (SDFs) ON A MICRO GLASS-PHONORECORD

Manipulation of DNA fibers is very useful technique for gene analysis such as Fluorescent In Situ Hybridization (FISH). In conventional methods for manipulation of DNA fibers, FISH efficiency often decreases due to the tight adhesion between DNA fibers and substrates. In this paper, we propose a micro glass-phonorecord with concentric grooves for suspending DNA fibers with centrifugal force. As a result of FISH analysis with the proposed technique, FISH efficiency of suspending DNA fibers was increased five times than one of the conventional stretching DNA fibers.